A novel, long-wavelength, non-fluorescent quencher (LQ), based on 1,4-diaminoanthraquinone, has been incorporated at the 3' and 5'-termini of oligonucleotides. The quencher has been used in Molecular beacons, efficiently quenching the long wavelength fluorophore, Cy5.
Keywords:
Carbocyanines
,Anthraquinones
,Oligonucleotide Probes
,Oligonucleotides
,Fluorescence Polarization
,Spectrometry, Fluorescence
,Genetic Techniques
