Escherichia coli Rsd protein was previously identified on the basis of its binding to the RNA polymerase sigma(70) subunit. The Rsd-sigma(70) complex has been studied using different methods. Our data show that Rsd associates with sigma(70) to form a complex with a stoichiometry of 1:1. Alanine scanning and deletion mutagenesis were used to locate regions of sigma(70) that are required for the formation of the Rsd-sigma(70) complex.
Keywords:
Binding Sites
,DNA-Directed RNA Polymerases
,Electrophoresis, Polyacrylamide Gel
,Escherichia coli Proteins
,Molecular Weight
,Mutagenesis, Site-Directed
,Protein Binding
,Repressor Proteins
,Sigma Factor
,Spectrometry, Mass, Electrospray Ionization
